Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas …

2008-03-10

(B) Phenotypes of abi5-1, ABI5 overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 μM), and AZD (1 μM) for 14 days. Supplementary Figure 3 | GUS staining of ABI5-GUS OE12 transgenic line. (A) GUS staining of leaves of 7-day-old ABI5-GUS OE12 plants treated with DMSO, ABA (50 μM), and AZD (2 μM) for 48 h. ABI5 is mainly expressed in dry seeds, and its expression markedly decreases after germination (Finkelstein and Lynch, 2000b; Lopez‐Molina et al., 2001). Expression of ABI5 is strongly induced by exogenous ABA (Lopez‐Molina et al., 2001). Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ-accumulating (JAZ-ΔJas) plants.

Abi5 overexpression

The mRNA and protein level of ABI5 in overexpression plants on WT and mutant backgrounds were examined by quantitative real‐time PCR and immunoblotting, respectively. Transgenic lines with similar amounts of ABI5 (En‐2 Super:ABI5‐MYC vs bes1‐D Super:ABI5‐MYC ) were selected as a pair, and two independent pairs were selected and used for the following studies (Fig. S2 a,b). Overexpression of RAV1 repressed ABI3, ABI4, and ABI5 expression, and RAV1 bound to the ABI3, ABI4, and ABI5 promoters in vitro and in vivo, indicating that RAV1 directly down-regulates the expression of ABI3, ABI4, and ABI5. Conversely, overexpression of ABI5 enhanced light‐mediated hypocotyl inhibition in seedlings (Chen et al., 2008).

Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis. Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell.

ABI5 was previously reported as the target protein of AFP2 during seed germination (Lopez-Molina et al., 2003), and overexpression of ABI5 activates the flowering negative factor FLC at the transcriptional level to repress flowering (Wang et al., 2013b). Thus, we compared the transcription of FLC in Col, afp2, and AFP2-ox under LD conditions. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent.

The overexpression of MdKNOX19 increased the ABA sensitivity of apple calli, resulting in a dramatic up-regulation in the transcription of the Arabidopsis ABI5 -like MdABI5 gene.

Noteworthily, we showed that down-regulation of NF-YC9 represses, but overexpression of NF-YC9 enhances, ABA-induced ABI5 expression (Fig.

It has been demonstrated that activity of ABI5 is transcriptionally and post‐translationally regulated. 2019-06-01 · However, the overexpression of ABI5 in the bes1-D background could not rescue the early-flowering phenotype . Because plants overexpressing ABI5 decreased the ABA insensitivity of bes1-D , it is indicated that ABI3-mediated negative regulation of the floral transition would be independent of ABI5 activity, at least under higher-BR-response conditions.
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Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants.

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Loss‐of‐function in ABI5 (abi5) promotes juvenile‐to‐adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent.

Moreover, the expression of eight other PYLs is … Loss‐of‐function in ABI5 (abi5) promotes juvenile‐to‐adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. ABI5 production is enhanced by stress including high salt and drought. This protects plants from drought. 2008-03-18 However, overexpression of ABI5 was not sufficient to repress germination, as ABI5 activity requires phosphorylation. The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line.